Quantifying E. coli Proteome and Transcriptome with Single-Molecule Sensitivity in Single Cells-Reference-Cited by-同舟云学术

Quantifying E. coli Proteome and Transcriptome with Single-Molecule Sensitivity in Single Cells

Published:2010-07-30 Issue:5991 Volume:329 Page:533-538
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Taniguchi Yuichi¹,Choi Paul J.¹,Li Gene-Wei¹²,Chen Huiyi¹³,Babu Mohan⁴,Hearn Jeremy¹,Emili Andrew⁴⁵,Xie X. Sunney¹

Affiliation:

1. Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.

2. Department of Physics, Harvard University, Cambridge, MA 02138, USA.

3. Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.

4. Banting and Best Department of Medical Research, Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto M5S 3E1, Canada.

5. Department of Molecular Genetics, University of Toronto, Toronto M5S 1A8, Canada.

Abstract

Devil in the Detail Genetically identical cells in the same environment can show variation in gene expression that may cause phenotypic variation at the single-cell level. But how noisy are most genes? Taniguchi et al. (p. 533 ; see the Perspective by

Tyagi

) now report single-cell global profiling of both messenger RNA (mRNA) and proteins in Escherichia coli using a yellow fluorescent protein fusion library. As well as a common extrinsic noise in high-abundance proteins, large fluctuations were observed in low-abundance proteins. Remarkably, in single-cell experiments, mRNA and protein levels for the same gene were uncorrelated.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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