Measuring Serotonin Distribution in Live Cells with Three-Photon Excitation-Reference-Cited by-同舟云学术

Measuring Serotonin Distribution in Live Cells with Three-Photon Excitation

Published:1997-01-24 Issue:5299 Volume:275 Page:530-532
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Maiti S.¹,Shear Jason B.¹,Williams R. M.¹,Zipfel W. R.¹,Webb Watt W.¹

Affiliation:

1. School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA. All authors contributed equally to this work.

Abstract

Tryptophan and serotonin were imaged with infrared illumination by three-photon excitation (3PE) of their native ultraviolet (UV) fluorescence. This technique, established by 3PE cross section measurements of tryptophan and the monoamines serotonin and dopamine, circumvents the limitations imposed by photodamage, scattering, and indiscriminate background encountered in other UV microscopies. Three-dimensionally resolved images are presented along with measurements of the serotonin concentration (∼50 mM) and content (up to ∼5 × 10 8 molecules) of individual secretory granules.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference29 articles.

1. Miyake K., McNeil P. L., J. Cell Biol. 131, 1737 (1995);

2. Ryan T. A. , Smith S. J. , Reuter H., , Proc. Natl. Acad. Sci. U.S.A. 93, 5567 (1996).

3. Spudich A., Braunstein D., Proc. Natl. Acad. Sci. U.S.A. 92, 6976 (1995).

4. De Matties M. A., De Tullio C., Buccione R., Luini A., J. Biol. Chem. 266, 10452 (1991);

5. Phillips D. M. , Bashkin P. , Pecht I., J. Ultrastruct. Res. 90, 105 (1985);

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