In situ genome sequencing resolves DNA sequence and structure in intact biological samples-Reference-Cited by-同舟云学术

In situ genome sequencing resolves DNA sequence and structure in intact biological samples

Published:2021-02-26 Issue:6532 Volume:371 Page:
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Payne Andrew C.¹²^ORCID,Chiang Zachary D.²³^ORCID,Reginato Paul L.¹²⁴⁵⁶^ORCID,Mangiameli Sarah M.²^ORCID,Murray Evan M.²,Yao Chun-Chen²⁷,Markoulaki Styliani⁸^ORCID,Earl Andrew S.³^ORCID,Labade Ajay S.³^ORCID,Jaenisch Rudolf⁸⁹^ORCID,Church George M.⁵⁶^ORCID,Boyden Edward S.¹⁴⁷¹⁰¹¹^ORCID,Buenrostro Jason D.²³^ORCID,Chen Fei²³^ORCID

Affiliation:

1. Media Arts and Sciences, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA.

2. Broad Institute of MIT and Harvard University, Cambridge, MA 02142, USA.

3. Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA.

4. Department of Biological Engineering, MIT, Cambridge, MA, 02139, USA.

5. Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.

6. Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA.

7. Department of Brain and Cognitive Sciences, MIT, Cambridge, MA 02139, USA.

8. Whitehead Institute for Biomedical Research, Cambridge, MA 02139, USA.

9. Department of Biology, MIT, Cambridge, MA 02139, USA.

10. McGovern Institute, MIT, Cambridge, MA 02139, USA.

11. Koch Institute, MIT, Cambridge, MA 02139, USA.

Abstract

Visualizing the 3D genome in situ The conformation of the genome within the cell changes depending on cell state, such that being able to visualize genome structure can identify cis and trans interactions among regulatory genetic elements. Payne et al. have developed an unbiased genome-sequencing technique in single cells in situ that can infer the chromatin structure by imaging. They were able to identify sequences at subnuclei locations to analyze the proximity relationships among genetic elements within and across chromosomes in single cells. Using this technique, they could detect chromosome territories and distinctions between different types of repetitive sequences and chromosomal features. This method can map and image genomic coordinates with submicrometer resolution in intact single cells. Science , this issue p. eaay3446

Funder

National Institutes of Health

Paul G. Allen Family Foundation

Natural Sciences and Engineering Research Council of Canada

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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