Inactivation of porcine endogenous retrovirus in pigs using CRISPR-Cas9-Reference-Cited by-同舟云学术

Inactivation of porcine endogenous retrovirus in pigs using CRISPR-Cas9

Published:2017-09-22 Issue:6357 Volume:357 Page:1303-1307
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Niu Dong¹²^ORCID,Wei Hong-Jiang³⁴^ORCID,Lin Lin⁵^ORCID,George Haydy¹,Wang Tao¹^ORCID,Lee I-Hsiu¹^ORCID,Zhao Hong-Ye³^ORCID,Wang Yong⁶,Kan Yinan¹,Shrock Ellen⁷,Lesha Emal¹^ORCID,Wang Gang¹^ORCID,Luo Yonglun⁵^ORCID,Qing Yubo³⁴^ORCID,Jiao Deling³⁴^ORCID,Zhao Heng³⁴^ORCID,Zhou Xiaoyang⁶,Wang Shouqi⁸^ORCID,Wei Hong⁶,Güell Marc¹^ORCID,Church George M.¹⁷⁹^ORCID,Yang Luhan¹^ORCID

Affiliation:

1. eGenesis, Cambridge, MA 02139, USA.

2. College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.

3. State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, China.

4. College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China.

5. Department of Biomedicine, Aarhus University, 8000 Aarhus C, Denmark.

6. Department of Laboratory Animal Science, College of Basic Medical Sciences, Third Military Medical University, Chongqing, 400038, China.

7. Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.

8. Research Institute of Shenzhen Jinxinnong Technology, Shenzhen 518106, China.

9. Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA 02138, USA.

Abstract

Taking the PERVs out of pigs With the severe shortage of organs needed for transplants, xenotransplantation (transplantation of nonhuman organs to humans) offers an alternative source. Some pig organs have similar size and function to those of humans. The challenge is that the pig genome harbors porcine endogenous retroviruses (PERVs) that can potentially pass to humans with possibly damaging consequences. Niu et al. generated pigs in which all copies of PERVs were inactivated by CRISPR-Cas9 genome engineering (see the Perspective by Denner). Not only does this work provide insights into PERV activity, but it also opens the door to a safer source of organs and tissues for pig-to-human xenotransplantation. Science , this issue p. 1303 ; see also p. 1238

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference15 articles.

1. Organ Shortage: The Greatest Challenge Facing Transplant Medicine

2. History of xenotransplantation

3. Infection of human cells by an endogenous retrovirus of pigs

4. How Active Are Porcine Endogenous Retroviruses (PERVs)?

5. Genome-wide inactivation of porcine endogenous retroviruses (PERVs)

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