Time-resolved crystallography captures light-driven DNA repair-Reference-Cited by-同舟云学术

Time-resolved crystallography captures light-driven DNA repair

Published:2023-12 Issue:6674 Volume:382 Page:1015-1020
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Christou Nina-Eleni¹^ORCID,Apostolopoulou Virginia¹²^ORCID,Melo Diogo V. M.³,Ruppert Matthias⁴^ORCID,Fadini Alisia⁵^ORCID,Henkel Alessandra¹^ORCID,Sprenger Janina¹^ORCID,Oberthuer Dominik¹^ORCID,Günther Sebastian¹^ORCID,Pateras Anastasios¹^ORCID,Rahmani Mashhour Aida¹^ORCID,Yefanov Oleksandr M.¹^ORCID,Galchenkova Marina¹^ORCID,Reinke Patrick Y. A.¹^ORCID,Kremling Viviane¹^ORCID,Scheer T. Emilie S.¹^ORCID,Lange Esther R.¹^ORCID,Middendorf Philipp¹^ORCID,Schubert Robin³^ORCID,De Zitter Elke⁶^ORCID,Lumbao-Conradson Koya⁷^ORCID,Herrmann Jonathan⁸,Rahighi Simin⁸,Kunavar Ajda⁹^ORCID,Beale Emma V.¹⁰^ORCID,Beale John H.¹⁰^ORCID,Cirelli Claudio¹⁰^ORCID,Johnson Philip J. M.¹⁰^ORCID,Dworkowski Florian¹⁰^ORCID,Ozerov Dmitry¹⁰^ORCID,Bertrand Quentin¹⁰^ORCID,Wranik Maximilian¹⁰^ORCID,Bacellar Camila¹⁰^ORCID,Bajt Saša¹²^ORCID,Wakatsuki Soichi⁸¹¹^ORCID,Sellberg Jonas A.¹²^ORCID,Huse Nils²⁴^ORCID,Turk Dušan¹³¹⁴^ORCID,Chapman Henry N.¹²¹⁵^ORCID,Lane Thomas J.¹²^ORCID

Affiliation:

1. Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany.

2. The Hamburg Centre for Ultrafast Imaging, Luruper Chaussee 149, 22761 Hamburg, Germany.

3. European XFEL GmbH, Holzkoppel 4, 22869 Schenefeld, Germany.

4. Institute for Nanostructure and Solid-State Physics, CFEL Universität Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany.

5. Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, London SW7 2AZ, UK.

6. Université Grenoble Alpes, CEA, CNRS, Institut de Biologie Structurale, 38000 Grenoble, France.

7. Linac Coherent Light Source, SLAC National Accelerator Laboratory, 2575 Sand Hill Rd, Menlo Park, CA 94025, USA.

8. Department of Structural Biology, Stanford University, 318 Campus Drive West, Stanford, CA 94305-5151, USA.

9. Laboratory for Fluid Dynamics and Thermodynamics, Faculty of Mechanical Engineering, University of Ljubljana, Aškerčeva 6, 1000 Ljubljana, Slovenia.

10. Paul Scherrer Institute, CH-5232 Villigen PSI, Switzerland.

11. Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, 2575 Sand Hill Rd, Menlo Park, CA 94025, USA.

12. Biomedical and X-ray Physics, Department of Applied Physics, AlbaNova University Center, KTH Royal Institute of Technology, S-106 91 Stockholm, Sweden.

13. Department of Biochemistry and Molecular and Structural Biology, Jožef Stefan Institute, Jamova 39, 1000 Ljubljana, Slovenia.

14. Centre of Excellence for Integrated Approaches in Chemistry and Biology of Proteins, Jamova 39, 1000 Ljubljana, Slovenia.

15. Department of Physics, Universität Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany.

Abstract

Photolyase is an enzyme that uses light to catalyze DNA repair. To capture the reaction intermediates involved in the enzyme’s catalytic cycle, we conducted a time-resolved crystallography experiment. We found that photolyase traps the excited state of the active cofactor, flavin adenine dinucleotide (FAD), in a highly bent geometry. This excited state performs electron transfer to damaged DNA, inducing repair. We show that the repair reaction, which involves the lysis of two covalent bonds, occurs through a single-bond intermediate. The transformation of the substrate into product crowds the active site and disrupts hydrogen bonds with the enzyme, resulting in stepwise product release, with the 3′ thymine ejected first, followed by the 5′ base.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Link

https://www.science.org/doi/pdf/10.1126/science.adj4270

Reference70 articles.

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2. Evolution of Mutation Rates: Phylogenomic Analysis of the Photolyase/Cryptochrome Family

3. Evolutionary History of the Photolyase/Cryptochrome Superfamily in Eukaryotes

4. Photocarcinogenesis: an overview

5. Mechanisms of DNA Repair by Photolyase and Excision Nuclease (Nobel Lecture)

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