Systematic Genetic Analysis with Ordered Arrays of Yeast Deletion Mutants-Reference-Cited by-同舟云学术

Systematic Genetic Analysis with Ordered Arrays of Yeast Deletion Mutants

Published:2001-12-14 Issue:5550 Volume:294 Page:2364-2368
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Tong Amy Hin Yan¹²,Evangelista Marie³,Parsons Ainslie B.¹²,Xu Hong¹²,Bader Gary D.⁴⁵,Pagé Nicholas⁶,Robinson Mark¹,Raghibizadeh Sasan⁷,Hogue Christopher W. V.⁴⁵,Bussey Howard⁶,Andrews Brenda²,Tyers Mike²⁵,Boone Charles¹²³

Affiliation:

1. Banting and Best Department of Medical Research, University of Toronto, Toronto ON, Canada M5G 1L6.

2. Department of Medical Genetics and Microbiology, University of Toronto, Toronto ON, Canada M5S 1A8.

3. Biology Department, Queens University, Kingston ON, Canada K7L 3N6.

4. Department of Biochemistry, University of Toronto, Toronto ON, Canada M5S 1A8.

5. Program in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mt. Sinai Hospital, Toronto ON, Canada M5G 1X5.

6. Department of Biology, McGill University, Montreal PQ, Canada H3A 1B1.

7. Virtek Engineering Sciences, Inc. (VESI), 1 Bedford Road, Toronto ON, Canada M5R 2J7.

Abstract

In Saccharomyces cerevisiae , more than 80% of the ∼6200 predicted genes are nonessential, implying that the genome is buffered from the phenotypic consequences of genetic perturbation. To evaluate function, we developed a method for systematic construction of double mutants, termed synthetic genetic array (SGA) analysis, in which a query mutation is crossed to an array of ∼4700 deletion mutants. Inviable double-mutant meiotic progeny identify functional relationships between genes. SGA analysis of genes with roles in cytoskeletal organization ( BNI1 , ARP2 , ARC40 , BIM1 ), DNA synthesis and repair ( SGS1 , RAD27 ), or uncharacterized functions ( BBC1 , NBP2 ) generated a network of 291 interactions among 204 genes. Systematic application of this approach should produce a global map of gene function.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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