Direct observation of motor protein stepping in living cells using MINFLUX-Reference-Cited by-同舟云学术

Direct observation of motor protein stepping in living cells using MINFLUX

Published:2023-03-10 Issue:6636 Volume:379 Page:1010-1015
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Deguchi Takahiro¹^ORCID,Iwanski Malina K.²^ORCID,Schentarra Eva-Maria¹³^ORCID,Heidebrecht Christopher¹³^ORCID,Schmidt Lisa¹³^ORCID,Heck Jennifer⁴^ORCID,Weihs Tobias⁵^ORCID,Schnorrenberg Sebastian⁶^ORCID,Hoess Philipp¹^ORCID,Liu Sheng¹⁷^ORCID,Chevyreva Veronika¹⁸^ORCID,Noh Kyung-Min⁴^ORCID,Kapitein Lukas C.²^ORCID,Ries Jonas¹^ORCID

Affiliation:

1. Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.

2. Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science, Utrecht University, Utrecht, Netherlands.

3. Faculty of Biosciences, University of Heidelberg, Heidelberg, Germany.

4. Genome Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.

5. Abberior Instruments GmbH, Göttingen, Germany.

6. EMBL Imaging Centre, European Molecular Biology Laboratory, Heidelberg, Germany.

7. Department of Physics and Astronomy, University of New Mexico, Albuquerque, NM, USA.

8. The FIRC Institute of Molecular Oncology, Milano, Italy.

Abstract

Dynamic measurements of molecular machines can provide invaluable insights into their mechanism, but these measurements have been challenging in living cells. Here, we developed live-cell tracking of single fluorophores with nanometer spatial and millisecond temporal resolution in two and three dimensions using the recently introduced super-resolution technique MINFLUX. Using this approach, we resolved the precise stepping motion of the motor protein kinesin-1 as it walked on microtubules in living cells. Nanoscopic tracking of motors walking on the microtubules of fixed cells also enabled us to resolve the architecture of the microtubule cytoskeleton with protofilament resolution.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Link

https://www.science.org/doi/pdf/10.1126/science.ade2676

Reference37 articles.

1. Movement of microtubules by single kinesin molecules

2. Myosin-V is a processive actin-based motor

3. Dynein arms are oscillating force generators

4. Direct observation of kinesin stepping by optical trapping interferometry

5. Germanium nanospheres for ultraresolution picotensiometry of kinesin motors

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