Cortical column and whole-brain imaging with molecular contrast and nanoscale resolution-Reference-Cited by-同舟云学术

Cortical column and whole-brain imaging with molecular contrast and nanoscale resolution

Published:2019-01-18 Issue:6424 Volume:363 Page:
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Gao Ruixuan¹²³^ORCID,Asano Shoh M.¹²^ORCID,Upadhyayula Srigokul³⁴⁵⁶^ORCID,Pisarev Igor³^ORCID,Milkie Daniel E.³^ORCID,Liu Tsung-Li³^ORCID,Singh Ved³,Graves Austin³,Huynh Grace H.¹^ORCID,Zhao Yongxin¹^ORCID,Bogovic John³^ORCID,Colonell Jennifer³,Ott Carolyn M.³,Zugates Christopher⁷^ORCID,Tappan Susan⁸^ORCID,Rodriguez Alfredo⁸,Mosaliganti Kishore R.⁹,Sheu Shu-Hsien³^ORCID,Pasolli H. Amalia³,Pang Song³,Xu C. Shan³^ORCID,Megason Sean G.⁹^ORCID,Hess Harald³^ORCID,Lippincott-Schwartz Jennifer³^ORCID,Hantman Adam³,Rubin Gerald M.³^ORCID,Kirchhausen Tom³⁴⁵⁶,Saalfeld Stephan³^ORCID,Aso Yoshinori³,Boyden Edward S.¹²¹⁰¹¹¹²^ORCID,Betzig Eric³¹³¹⁴¹⁵¹⁶^ORCID

Affiliation:

1. MIT Media Lab, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA.

2. McGovern Institute for Brain Research, MIT, Cambridge, MA 02139, USA.

3. Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.

4. Department of Cell Biology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA.

5. Program in Cellular and Molecular Medicine, Boston Children’s Hospital, 200 Longwood Avenue, Boston, MA 02115, USA.

6. Department of Pediatrics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA.

7. arivis AG, 1875 Connecticut Avenue NW, 10th floor, Washington, DC 20009, USA.

8. MBF Bioscience, 185 Allen Brook Lane, Suite 101, Williston, VT 05495, USA.

9. Department of Systems Biology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA.

10. Department of Biological Engineering, MIT, Cambridge, MA 02139, USA.

11. MIT Center for Neurobiological Engineering, MIT, Cambridge, MA 02139, USA.

12. Department of Brain and Cognitive Sciences, MIT, Cambridge, MA 02139, USA.

13. Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.

14. Department of Physics, University of California, Berkeley, CA 94720, USA.

15. Howard Hughes Medical Institute, Berkeley, CA 94720, USA.

16. Helen Wills Neuroscience Institute, Berkeley, CA 94720, USA.

Abstract

Combining expansion and the lattice light sheet Optical and electron microscopy have made tremendous inroads into understanding the complexity of the brain. Gao et al. introduce an approach for high-resolution tracing of neurons, their subassemblies, and their molecular constituents over large volumes. They applied their method, which combines expansion microscopy and lattice light-sheet microscopy, to the mouse cortical column and the entire Drosophila brain. The approach can be performed at speeds that should enable high-throughput comparative studies of neural development, circuit stereotypy, and structural correlations to neural activity or behavior. Science , this issue p. eaau8302

Funder

National Institutes of Health

Howard Hughes Medical Institute

IARPA

US-Israel Binational Science Foundation

John Doerr

The Open Philanthropy Project

HHMI-Simons Faculty Scholars Program

U. S. Army Research Laboratory and U. S. Army Research Office

Publisher

American Association for the Advancement of Science (AAAS)

Subject