Long-term functional maintenance of primary human hepatocytes in vitro-Reference-Cited by-同舟云学术

Long-term functional maintenance of primary human hepatocytes in vitro

Published:2019-04-26 Issue:6438 Volume:364 Page:399-402
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Xiang Chengang¹^ORCID,Du Yuanyuan¹^ORCID,Meng Gaofan¹^ORCID,Soon Yi Liew¹,Sun Shicheng¹,Song Nan¹,Zhang Xiaonan²^ORCID,Xiao Yiwei³^ORCID,Wang Jie³,Yi Zhigang⁴,Liu Yifang⁵,Xie Bingqing⁶^ORCID,Wu Min²,Shu Jun⁴,Sun Da⁶,Jia Jun¹,Liang Zhen⁶,Sun Dong¹,Huang Yanxiang⁷,Shi Yan¹^ORCID,Xu Jun¹,Lu Fengmin³,Li Cheng⁵,Xiang Kuanhui³,Yuan Zhenghong⁴,Lu Shichun⁸^ORCID,Deng Hongkui¹⁶^ORCID

Affiliation:

1. School of Basic Medical Sciences, State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center and the MOE Key Laboratory of Cell Proliferation and Differentiation, College of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100191, China.

2. Shanghai Public Health Clinical Center, Shanghai 201508, China.

3. School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.

4. Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai 200032, China.

5. Center for Bioinformatics, Peking University, Beijing 100871, China.

6. State Key Laboratory of Chemical Oncogenomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, Shenzhen 518055, Guangdong, China.

7. Youan Hospital, Beijing 100069, China.

8. Department of Hepatobiliary Surgery, Chinese PLA General Hospital, Beijing 100853, China.

Abstract

Keep hepatocytes fresh Freshly isolated primary human hepatocytes (PHHs) quickly lose their identity and function in vitro, limiting their application in modeling infectious liver diseases and screening drugs. Xiang et al. describe a simple and effective five-chemical culture condition that can maintain the mature function of cultured PHHs for an extended period of time. PHHs cultured in this way can support the entire viral life cycle and recapitulate long-term infection of the hepatitis B virus. This system created a useful drug-screening platform for developing new antiviral strategies. Science , this issue p. 399

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference18 articles.

1. Competency of different cell models to predict human hepatotoxic drugs

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3. Present and future therapies of hepatitis B: From discovery to cure

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