Structure of a yeast spliceosome at 3.6-angstrom resolution

Author:

Yan Chuangye1,Hang Jing1,Wan Ruixue1,Huang Min2,Wong Catherine C. L.2,Shi Yigong1

Affiliation:

1. Ministry of Education Key Laboratory of Protein Science, Tsinghua-Peking Joint Center for Life Sciences, Center for Structural Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.

2. National Center for Protein Science Shanghai, Institute of Biochemistry and Cell Biology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.

Abstract

Structure and function of the spliceosome When RNA is transcribed from DNA in the eukaryotic cell nucleus, the initial transcript includes noncoding introns that must be spliced out. This splicing is done by a complex macromolecular machine, the spliceosome, which comprises five small nuclear RNAs and more than 100 associated proteins. Now, two papers reveal insights into the structure and function of the yeast spliceosome. Yan et al. describe a high-resolution structure determined by electron microscopy of a spliceosome complex comprising four RNAs and 37 proteins. Hang et al. focus on the catalytic site and show how protein components anchor the transcribed RNA and allow sufficient flexibility to deliver RNA components involved in catalyzing the splicing reaction. Science , this issue pp. 1182 and 1191

Funder

National Natural Science Foundation of China

China's Ministry of Science and Technology

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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