ChromEMT: Visualizing 3D chromatin structure and compaction in interphase and mitotic cells-Reference-Cited by-全球学者库

ChromEMT: Visualizing 3D chromatin structure and compaction in interphase and mitotic cells

Published:2017-07-28 Issue:6349 Volume:357 Page:
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Ou Horng D.¹^ORCID,Phan Sébastien²^ORCID,Deerinck Thomas J.²,Thor Andrea²,Ellisman Mark H.²³,O’Shea Clodagh C.¹^ORCID

Affiliation:

1. Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.

2. National Center for Microscopy and Imaging Research, Center for Research in Biological Systems, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.

3. Department of Neurosciences, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.

Abstract

A close-up view inside the nucleus The nuclei of human cells contain 2 meters of genomic DNA. How does it all fit? Compaction starts with the DNA wrapping around histone octamers to form nucleosomes, but it is unclear how these further compress into mitotic chromosomes. Ou et al. describe a DNA-labeling method that allows them to visualize chromatin organization in human cells (see the Perspective by Larson and Misteli). They show that chromatin forms flexible chains with diameters between 5 and 24 nm. In mitotic chromosomes, chains bend back on themselves to pack at high density, whereas during interphase, the chromatin chains are more extended. Science , this issue p. eaag0025 ; see also p. 354

Funder

Howard Hughes Medical Institute

NIH Office of the Director

National Cancer Institute

National Institute of General Medical Sciences

W.M. Keck Foundation

Leona M. and Harry B. Helmsley Charitable Trust

Louis and Harold Price Foundation

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary