In Situ Visualization of DNA Double-Strand Break Repair in Human Fibroblasts-Reference-Cited by-同舟云学术

In Situ Visualization of DNA Double-Strand Break Repair in Human Fibroblasts

Published:1998-04-24 Issue:5363 Volume:280 Page:590-592
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Nelms Benjamin E.¹²³,Maser Richard S.¹²³,MacKay James F.¹²³,Lagally Max G.¹²³,Petrini John H. J.¹²³

Affiliation:

1. B. E. Nelms, Laboratory of Genetics and Department of Medical Physics, University of Wisconsin Medical School, Madison, WI 53706, USA.

2. R. S. Maser and J. H. J. Petrini, Laboratory of Genetics, University of Wisconsin Medical School, Madison, WI 53706, USA.

3. J. F. MacKay and M. G. Lagally, Department of Materials Science and Engineering, University of Wisconsin, Madison, WI 53706, USA.

Abstract

A method was developed to examine DNA repair within the intact cell. Ultrasoft x-rays were used to induce DNA double-strand breaks (DSBs) in defined subnuclear volumes of human fibroblasts and DNA repair was visualized at those sites. The DSBs remained in a fixed position during the initial stages of DNA repair, and the DSB repair protein hMre11 migrated to the sites of damage within 30 minutes. In contrast, hRad51, a human RecA homolog, did not localize at sites of DNA damage, a finding consistent with the distinct roles of these proteins in DNA repair.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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