Chemical Transformations in Individual Ultrasmall Biomimetic Containers-Reference-Cited by-同舟云学术

Chemical Transformations in Individual Ultrasmall Biomimetic Containers

Published:1999-03-19 Issue:5409 Volume:283 Page:1892-1895
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Chiu Daniel T.¹,Wilson Clyde F.¹,Ryttsén Frida²,Strömberg Anette²,Farre Cecilia²,Karlsson Anders²,Nordholm Sture²,Gaggar Anuj¹,Modi Biren P.¹,Moscho Alexander¹,Garza-López Roberto A.³,Orwar Owe²,Zare Richard N.¹

Affiliation:

1. Department of Chemistry, Stanford University, Stanford, CA 94305, USA.

2. Department of Chemistry, Göteborg University, Göteborg, SE-41296, Sweden.

3. Department of Chemistry, Pomona College, Claremont, CA 91711, USA.

Abstract

Individual phospholipid vesicles, 1 to 5 micrometers in diameter, containing a single reagent or a complete reaction system, were immobilized with an infrared laser optical trap or by adhesion to modified borosilicate glass surfaces. Chemical transformations were initiated either by electroporation or by electrofusion, in each case through application of a short (10-microsecond), intense (20 to 50 kilovolts per centimeter) electric pulse delivered across ultramicroelectrodes. Product formation was monitored by far-field laser fluorescence microscopy. The ultrasmall characteristic of this reaction volume led to rapid diffusional mixing that permits the study of fast chemical kinetics. This technique is also well suited for the study of reaction dynamics of biological molecules within lipid-enclosed nanoenvironments that mimic cell membranes.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference34 articles.

1. We used a Brownian dynamics simulation program in which we treated a single enzyme and a single substrate as hard spheres. The radius of each molecule was estimated from the Stokes–Einstein law as described [

2. Ermak D. L., J. Chem. Phys. 62, 4189 (1975);

3. ]. The diffusion constants for the molecules were modeled by changing the time steps taken by the molecules and the constant velocities given to them. The trajectories were followed and the diffusion constants were calculated as described [

4. Turq P., Lantalme F., Friedman H. L., J. Chem. Phys. 66, 3039 (1977);

5. ]. We used D (enzyme) = 7 × 10 −11 m 2 s −1 and D (substrate) = 4.4 × 10 −10 m 2 s −1 . This model was later used to obtain an estimate of the number of collisions between enzyme and substrate and also between the different molecules and the phospholipid wall which was treated as a hard wall. Substrate-wall collisions scale as 1/ r and substrate-enzyme collisions scale as 1/ r 3 where r is the vesicle radius.

Cited by 240 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Measurements of Spontaneous and External Stimuli Molecular Release Processes from a Single Optically Trapped Poly(lactic-co-glycolic) Acid Microparticle and a Liposome Containing Gold Nanospheres;Analytical Chemistry;2024-07-30

2. Lipid vesicle-based molecular robots;Lab on a Chip;2024

3. Interlinking spatial dimensions and kinetic processes in dissipative materials to create synthetic systems with lifelike functionality;Nature Nanotechnology;2023-12-06

4. Single-molecule immunoassay technology: Recent advances;Talanta;2023-12

5. Micro- and nanochamber array system for single enzyme assays;Scientific Reports;2023-08-16