Visualizing tmRNA Entry into a Stalled Ribosome-Reference-Cited by-同舟云学术

Visualizing tmRNA Entry into a Stalled Ribosome

Published:2003-04-04 Issue:5616 Volume:300 Page:127-130
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Valle Mikel¹,Gillet Reynald²,Kaur Sukhjit¹,Henne Anke³,Ramakrishnan V.²,Frank Joachim¹⁴

Affiliation:

1. Howard Hughes Medical Institute, Wadsworth Center, Health Research, Inc., Empire State Plaza, Albany, NY 12201–0509, USA.

2. MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK.

3. Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, Grisebachstrasse 8, 37077 Göttingen, Germany.

4. Department of Biomedical Sciences, State University of New York at Albany, Empire State Plaza, Albany, New York 12201–0509, USA.

Abstract

Bacterial ribosomes stalled on defective messenger RNAs (mRNAs) are rescued by tmRNA, an ∼300-nucleotide-long molecule that functions as both transfer RNA (tRNA) and mRNA. Translation then switches from the defective message to a short open reading frame on tmRNA that tags the defective nascent peptide chain for degradation. However, the mechanism by which tmRNA can enter and move through the ribosome is unknown. We present a cryo–electron microscopy study at ∼13 to 15 angstroms of the entry of tmRNA into the ribosome. The structure reveals how tmRNA could move through the ribosome despite its complicated topology and also suggests roles for proteins S1 and SmpB in the function of tmRNA.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference42 articles.

1. The SsrA–SmpB system for protein tagging, directed degradation and ribosome rescue

2. Emerging views on tmRNA-mediated protein tagging and ribosome rescue

3. The biological roles of trans-translation

4. Role of a Peptide Tagging System in Degradation of Proteins Synthesized from Damaged Messenger RNA

5. SsrA-mediated peptide tagging caused by rare codons and tRNA scarcity

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