Multiplex single-cell profiling of chromatin accessibility by combinatorial cellular indexing-Reference-Cited by-同舟云学术

Multiplex single-cell profiling of chromatin accessibility by combinatorial cellular indexing

Published:2015-05-22 Issue:6237 Volume:348 Page:910-914
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Cusanovich Darren A.¹,Daza Riza¹,Adey Andrew²,Pliner Hannah A.¹,Christiansen Lena³,Gunderson Kevin L.³,Steemers Frank J.³,Trapnell Cole¹,Shendure Jay¹

Affiliation:

1. University of Washington, Department of Genome Sciences, Seattle, WA, USA.

2. Oregon Health and Science University, Department of Molecular and Medical Genetics, Portland, OR, USA.

3. Illumina, Inc., Advanced Research Group, San Diego, CA, USA.

Abstract

Chromatin state and the single cell Identifying the chromatin state of any single cell, which may or may not have a different function or represent different stages relative to others collected within any single culture, experiment, or tissue, has been challenging. Cusanovitch et al. skirted previously identified technological limitations to identify regions of accessible chromatin at single-cell resolution. Combinatorial cellular indexing, a strategy for multiplex barcoding of thousands of single cells per experiment, was successfully used to investigate the genome-wide chromatin accessibility landscape in each of over 15,000 single cells. Science , this issue p. 910

Funder

NIH

Paul G. Allen Family Foundation

Damon Runyon Cancer Research Foundation

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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