Watching a Protein as it Functions with 150-ps Time-Resolved X-ray Crystallography-Reference-Cited by-同舟云学术

Watching a Protein as it Functions with 150-ps Time-Resolved X-ray Crystallography

Published:2003-06-20 Issue:5627 Volume:300 Page:1944-1947
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Schotte Friedrich¹²³⁴⁵,Lim Manho¹²³⁴⁵,Jackson Timothy A.¹²³⁴⁵,Smirnov Aleksandr V.¹²³⁴⁵,Soman Jayashree¹²³⁴⁵,Olson John S.¹²³⁴⁵,Phillips George N.¹²³⁴⁵,Wulff Michael¹²³⁴⁵,Anfinrud Philip A.¹²³⁴⁵

Affiliation:

1. Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA.

2. Department of Chemistry, Pusan National University, Pusan, 609-735, Korea.

3. Harvard Medical School, Boston, MA 02115, USA.

4. Department of Biochemistry and Cell Biology, Rice University, Houston, TX 77251–1892, USA.

5. Biochemistry Department, University of Wisconsin, Madison, WI 53706, USA.

Abstract

We report picosecond time-resolved x-ray diffraction from the myoglobin (Mb) mutant in which Leu 29 is replaced by Phe (L29Fmutant). The frame-by-frame structural evolution, resolved to 1.8 angstroms, allows one to literally “watch” the protein as it executes its function. Time-resolved mid-infrared spectroscopy of flash-photolyzed L29F MbCO revealed a short-lived CO intermediate whose 140-ps lifetime is shorter than that found in wild-type protein by a factor of 1000. The electron density maps of the protein unveil transient conformational changes far more dramatic than the structural differences between the carboxy and deoxy states and depict the correlated side-chain motion responsible for rapidly sweeping CO away from its primary docking site.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference25 articles.

1. The Protein Data Bank

2. R. H. Austin, K. W. Beeson, L. Eisenstein, H. Frauenfelder, I. C. Gunsalus, Biochemistry14, 5355 (1975).

3. A. Ansari, C. M. Jones, E. R. Henry, J. Hofrichter, W. A. Eaton, Biochemistry33, 5128 (1994).

4. Complex nonexponential relaxation in myoglobin after photodissociation of MbCO: measurement and analysis from 2 ps to 56 υs

5. X. Ye, A. Demidov, P. M. Champion, J. Am. Chem. Soc.124, 5914 (2002).

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