Affiliation:
1. Department of Genetics and
2. Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755–3844, USA.
Abstract
In
Neurospora crassa
, white collar 1 (WC-1), a transcriptional activator and positive clock element, is rhythmically expressed from a nonrhythmic steady-state pool of
wc-1
transcript, consistent with posttranscriptional regulation of rhythmicity. Mutations in
frq
influence both the level and periodicity of WC-1 expression, and driven FRQ expression not only depresses its own endogenous levels, but positively regulates WC-1 synthesis with a lag of about 8 hours, a delay similar to that seen in the wild-type clock. FRQ thus plays dual roles in the
Neurospora
clock and thereby, with WC-1, forms a second feedback loop that would promote robustness and stability in this circadian system. The existence also of interlocked loops in
Drosophila melanogaster
and mouse clocks suggests that such interlocked loops may be a conserved aspect of circadian timing systems.
Publisher
American Association for the Advancement of Science (AAAS)
Cited by
318 articles.
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