Response to Comment on “Global diversity and geography of soil fungi”-Reference-Cited by-同舟云学术

Response to Comment on “Global diversity and geography of soil fungi”

Published:2015-08-28 Issue:6251 Volume:349 Page:936-936
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Tedersoo Leho¹,Bahram Mohammad²³,Põlme Sergei¹,Anslan Sten²,Riit Taavi²,Kõljalg Urmas²,Nilsson R. Henrik⁴,Hildebrand Falk⁵,Abarenkov Kessy¹

Affiliation:

1. Natural History Museum, University of Tartu, Tartu, Estonia.

2. Institute of Ecology and Earth Sciences, University of Tartu, Tartu, Estonia.

3. Department of Organismal Biology, Uppsala University, Uppsala, Sweden.

4. Department of Biological and Environmental Sciences, University of Gothenburg, Göteborg, Sweden.

5. European Molecular Biology Laboratory, Heidelberg, Germany.

Abstract

Schadt and Rosling (Technical Comment, 26 June 2015, p. 1438) argue that primer-template mismatches neglected the fungal class Archaeorhizomycetes in a global soil survey. Amplicon-based metabarcoding of nine barcode-primer pair combinations and polymerase chain reaction (PCR)–free shotgun metagenomics revealed that barcode and primer choice and PCR bias drive the diversity and composition of microorganisms in general, but the Archaeorhizomycetes were little affected in the global study. We urge that careful choice of DNA markers and primers is essential for ecological studies using high-throughput sequencing for identification.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference11 articles.

1. Comment on “Global diversity and geography of soil fungi”

2. Shotgun metagenomes and multiple primer pair-barcode combinations of amplicons reveal biases in metabarcoding analyses of fungi

3. Archaeorhizomycetes: Unearthing an Ancient Class of Ubiquitous Soil Fungi

4. Archaeorhizomyces borealis sp. nov. and a sequence-based classification of related soil fungal species

5. Global diversity and geography of soil fungi;Tedersoo L.;Science,2014

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