Structures of the CRISPR-Cmr complex reveal mode of RNA target positioning-Reference-Cited by-同舟云学术

Structures of the CRISPR-Cmr complex reveal mode of RNA target positioning

Published:2015-05 Issue:6234 Volume:348 Page:581-585
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Taylor David W.¹²,Zhu Yifan³,Staals Raymond H. J.³,Kornfeld Jack E.¹,Shinkai Akeo⁴⁵,van der Oost John³,Nogales Eva¹²⁶⁷,Doudna Jennifer A.¹²⁶⁸⁹

Affiliation:

1. Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA.

2. California Institute for Quantitative Biosciences, University of California, Berkeley, CA 94720, USA.

3. Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, 6703 HB Wageningen, Netherlands.

4. RIKEN SPring-8 Center, Hyogo 679-5148, Japan.

5. RIKEN Structural Biology Laboratory, Kanagawa 230-0045, Japan.

6. Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.

7. Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

8. Department of Chemistry, University of California, Berkeley, CA 94720, USA.

9. Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

Abstract

Changing shape to destroy RNA Clustered regularly interspaced short palindromic repeats (CRISPRs) together with CRISPR-associated (Cas) proteins form an adaptive immune system that helps bacteria and archaea defend themselves against invading viruses and plasmids. CRISPR RNAs (crRNAs) target CRISPR-Cas protein complexes to the invaders, bringing about their destruction. Taylor et al. used cryo–electron microscopy to determine the structure of a 12-subunit CRISPR-Cas protein complex with crRNA from Thermus thermophilus , in the presence and absence of single-stranded target RNA. Binding to the target RNA causes a change in shape of the CRISPR-Cas complex that results in target recognition and destruction. Science , this issue p. 581

Funder

Howard Hughes Medical Institute

Damon Runyon Cancer Research Foundation

Japan Society for the Promotion of Science

Netherlands Organisation for Scientific Research

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference29 articles.

1. RNA-guided genetic silencing systems in bacteria and archaea

2. CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes