Bakers' Yeast, a Model for Fungal Biofilm Formation-Reference-Cited by-同舟云学术

Bakers' Yeast, a Model for Fungal Biofilm Formation

Published:2001-02-02 Issue:5505 Volume:291 Page:878-881
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Reynolds Todd B.¹,Fink Gerald R.¹

Affiliation:

1. Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology, Nine Cambridge Center, Cambridge, MA 02142, USA.

Abstract

Biofilms are formed by the aggregation of microorganisms into multicellular structures that adhere to surfaces. Here we show that bakers' yeast Saccharomyces cerevisiae can initiate biofilm formation. When grown in low-glucose medium, the yeast cells adhered avidly to a number of plastic surfaces. On semi-solid (0.3% agar) medium they formed “mats”: complex multicellular structures composed of yeast-form cells. Both attachment to plastic and mat formation require Flo11p, a member of a large family of fungal cell surface glycoproteins involved in adherence. The ability to study biofilm formation in a tractable genetic system may facilitate the identification of new targets for antifungal therapy.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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5. For visualization of biofilms in wells of polystyrene plates a protocol was adapted from G. A. O'Toole et al. [ Methods Enzymol. 310 91 (1999)]; an equal volume of a solution of 1% (w/v) crystal violet was added to the cells and left for at least 15 min. The wells were washed repeatedly with H 2 0 and then photographed. Cells were also observed to adhere to polystyrene when not stained with crystal violet.

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