Structure of Tetrahymena telomerase reveals previously unknown subunits, functions, and interactions-Reference-Cited by-同舟云学术

Structure of Tetrahymena telomerase reveals previously unknown subunits, functions, and interactions

Published:2015-10-30 Issue:6260 Volume:350 Page:
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Jiang Jiansen¹²³,Chan Henry¹,Cash Darian D.¹,Miracco Edward J.¹,Ogorzalek Loo Rachel R.⁴,Upton Heather E.⁵,Cascio Duilio¹⁶,O’Brien Johnson Reid¹,Collins Kathleen⁵,Loo Joseph A.¹⁴⁶,Zhou Z. Hong²³,Feigon Juli¹³⁶

Affiliation:

1. Department of Chemistry and Biochemistry, University of California, Los Angeles (UCLA), Los Angeles, CA 90095, USA.

2. Department of Microbiology, Immunology, and Molecular Genetics, UCLA, Los Angeles, CA 90095, USA.

3. California Nanosystems Institute, UCLA, Los Angeles, CA 90095, USA.

4. Department of Biological Chemistry, UCLA, Los Angeles, CA 90095, USA.

5. Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.

6. UCLA–U.S. Department of Energy (DOE) Institute of Genomics and Proteomics, UCLA, Los Angeles, CA 90095, USA.

Abstract

Chromosome-capping enzyme complex Telomeres cap and protect the ends of our chromosomes. The telomerase complex helps maintain the telomere DNA repeat sequences. Telomerase consists of an RNA and a number of protein subunits. Jiang et al. used cryo–electron microscopy and x-ray crystallography to determine the structure of the Tetrahymena telomerase complex. The telomerase is made up of three subcomplexes, which include two previously unknown protein subunits in addition to the seven known subunits. The structures also reveal the path of the RNA component in the telomerase catalytic core. Science , this issue p. 10.1126/science.aab4070

Funder

NSF

NIH

DOE

American Heart Association

NIH Ruth L. Kirschstein National Research Service Award (NRSA)

NIH Ruth L. Kirschstein NRSA

National Center for Advancing Translational Sciences UCLA Clinical and Translational Science Institute

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary