A DNA Microarray-Based Genetic Screen for Nonhomologous End-Joining Mutants in Saccharomyces cerevisiae-Reference-Cited by-同舟云学术

A DNA Microarray-Based Genetic Screen for Nonhomologous End-Joining Mutants in Saccharomyces cerevisiae

Published:2001-12-21 Issue:5551 Volume:294 Page:2552-2556
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Ooi Siew Loon¹,Shoemaker Daniel D.²,Boeke Jef D.¹

Affiliation:

1. Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, 617 Hunterian Building, 725 North Wolfe Street, Baltimore, MD 21205, USA.

2. Rosetta Inpharmatics Inc., 12040 115th Street Avenue Northeast, Kirkland, WA 98034, USA.

Abstract

We describe a microarray-based screen performed by imposing different genetic selections on thousands of yeast mutants in parallel, representing most genes in the yeast genome. The presence or absence of mutants was detected by oligonucleotide arrays that hybridize to 20-nucleotide “barcodes.” We used this method to screen for components of the nonhomologous end-joining (NHEJ) pathway. Known components of the pathway were identified, as well as a gene not previously known to be involved in NHEJ, NEJ1 . Nej1 protein interacts with the amino terminus of LIF1/XRCC4, a recently recognized “guardian of the genome” against cancer.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference27 articles.

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4. Genomic profiling of drug sensitivities via induced haploinsufficiency

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