A Combined Experimental and Computational Strategy to Define Protein Interaction Networks for Peptide Recognition Modules-Reference-Cited by-同舟云学术

A Combined Experimental and Computational Strategy to Define Protein Interaction Networks for Peptide Recognition Modules

Published:2002-01-11 Issue:5553 Volume:295 Page:321-324
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Tong Amy Hin Yan¹,Drees Becky²,Nardelli Giuliano³,Bader Gary D.⁴,Brannetti Barbara³,Castagnoli Luisa³,Evangelista Marie⁵,Ferracuti Silvia³,Nelson Bryce⁵,Paoluzi Serena³,Quondam Michele³,Zucconi Adriana³,Hogue Christopher W. V.⁴,Fields Stanley²⁶,Boone Charles¹⁵,Cesareni Gianni³

Affiliation:

1. Banting and Best Department of Medical Research and Department of Molecular and Medical Genetics, University of Toronto, Toronto, Ontario, Canada M5G 1L6.

2. Department of Genome Sciences and Department of Medicine, University of Washington, Box 357730, Seattle, WA 98195, USA.

3. Department of Biology, University of Rome Tor Vergata, Via Della Ricerca Scientifica, 00133 Rome, Italy.

4. Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario, Canada M5S 1A8 and Department of Biochemistry, University of Toronto, Ontario, Canada M5G 1L6.

5. Department of Biology, Queens University, Kingston, Ontario, Canada K7L 3N6.

6. Howard Hughes Medical Institute, University of Washington, Box 353360, Seattle, WA 98195, USA.

Abstract

Peptide recognition modules mediate many protein-protein interactions critical for the assembly of macromolecular complexes. Complete genome sequences have revealed thousands of these domains, requiring improved methods for identifying their physiologically relevant binding partners. We have developed a strategy combining computational prediction of interactions from phage-display ligand consensus sequences with large-scale two-hybrid physical interaction tests. Application to yeast SH3 domains generated a phage-display network containing 394 interactions among 206 proteins and a two-hybrid network containing 233 interactions among 145 proteins. Graph theoretic analysis identified 59 highly likely interactions common to both networks. Las17 (Bee1), a member of the Wiskott-Aldrich Syndrome protein (WASP) family of actin-assembly proteins, showed multiple SH3 interactions, many of which were confirmed in vivo by coimmunoprecipitation.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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