Affiliation:
1. Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
Abstract
In
Saccharomyces cerevisiae,
the telomerase components Est2p,
TLC1
RNA, Est1p, and Est3p are thought to form a complex that acts late during chromosome replication (S phase) upon recruitment by Cdc13p, a telomeric DNA binding protein. Consistent with this model, we show that Est1p, Est2p, and Cdc13p are telomere-associated at this time. However, Est2p, but not Est1p, also binds telomeres before late S phase. The
cdc13-2
allele has been proposed to be defective in recruitment, yet Est1p and Est2p telomere association persists in
cdc13-2
cells. These findings suggest a model in which Est1p binds telomeres late in S phase and interacts with Cdc13p to convert inactive, telomere-bound Est2p to an active form.
Publisher
American Association for the Advancement of Science (AAAS)
Cited by
309 articles.
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