A Potassium Channel Protein Encoded by Chlorella Virus PBCV-1-Reference-Cited by-同舟云学术

A Potassium Channel Protein Encoded by Chlorella Virus PBCV-1

Published:2000-03-03 Issue:5458 Volume:287 Page:1641-1644
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Plugge B.¹,Gazzarrini S.²,Nelson M.³,Cerana R.⁴,Van J. L.,Etten ⁵,Derst C.⁶,DiFrancesco D.²,Moroni A.²,Thiel G.¹

Affiliation:

1. Albrecht-von-Haller-Institut für Pflanzenwissenschaften, Universität Göttingen, 37073 Göttingen, Germany.

2. Università degli Studi di Milano, 20133 Milan, Italy.

3. Megabase Research Products, 4711 Huntington Avenue, Lincoln, NE 68604, USA.

4. DISAT, Università di Milano Bicocca, 20126 Milan, Italy.

5. Department of Plant Pathology, University of Nebraska, Lincoln, NE 68583–0722, USA.

6. Institut für Physiologie, Philipps-Universität Marburg, 35033 Marburg, Germany.

Abstract

The large chlorella virus PBCV-1, which contains double-stranded DNA (dsDNA), encodes a 94-codon open reading frame (ORF) that contains a motif resembling the signature sequence of the pore domain of potassium channel proteins. Phylogenetic analyses of the encoded protein, Kcv, indicate a previously unidentified type of potassium channel. The messenger RNA encoded by the ORF leads to functional expression of a potassium-selective conductance in Xenopus laevis oocytes. The channel blockers amantadine and barium, but not cesium, inhibit this conductance, in addition to virus plaque formation. Thus, PBCV-1 encodes the first known viral protein that functions as a potassium-selective channel and is essential in the virus life cycle.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference42 articles.

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3. Single-letter abbreviations for the amino acid residues are as follows: A Ala; C Cys; D Asp; E Glu; F Phe; G Gly; H His; I Ile; K Lys; L Leu; M Met; N Asn; P Pro; Q Gln; R Arg; S Ser; T Thr; V Val; W Trp; and Y Tyr. X indicates any residue.

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