Affiliation:
1. Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences
Abstract
The effect of cavitated sunflower husk and lactic acid on the changes in the taxonomic profile of the rumen microbiome of ruminants in vitro was evaluated. Changes in the bacterial composition of the cicatricial fluid were determined by using the MiSeq sequencing method in a time exposure of 6-24 h. The objects of research were sunflower husk milled (0.01 cm) - control; cavitated: husk and water in a ratio of 1 : 3, treated with ultrasound; husk and lactic acid in a ratio of 1 : 3 - cavitated husk + lactic acid. The obtained samples after drying at 105 ºC were used in in vitro studies with rumen fluid on an incubator (analog of artificial rumen). Selection of the rumen fluid in cattle was carried out through a chronic fistula. The age of animals - 12 months, the breed - Kazakh white-headed. Administration of cavitated sunflower husks alone or together with lactic acid after 6 h increased the number of bacteria belonging to the Flavobacteriia class by 5.43 and 3.93%, Elusimicrobia by 3.77 and 3.98%, Gammaproteobacteria by 11.75 and 10.07% and decreased the number of Bacteroidia by 5.72 and 2.63%, Clostridia by 3.26 and 2.20% relative to the control. In contrast, in the 12-24 h exposure time, there was an increase in Bacteroidia class bacteria by 3.30-4.90 and 8.29-11.28%, Clostridia by 1.66-1.50 and 2.74-3.27% relative to the control.