Effects of Hydroquinone on Cytoskeletal Organization and Intracellular Transport in Cultured Xenopus laevis Melanophores and Fibroblasts

Abstract

Hydroquinone is used as a skin-lightening agent, it is also present in different chemical products and cigarette smoke. It is believed to inhibit melanin production in melanocytes by inhibiting the key enzyme tyrosinase. In the present study, we show that hydroquinone had severe effects on microtubules and actin filaments in cultured Xenopus laevis melanophores as studied by immunohistochemistry. It affected the intracellular transport of melanosomes, induced bundling of microtubules and disassembly of actin filaments at 10 and 50 μM, and at 100 μM proper adhesion to the substrate was lost. Effects occurred at lower concentrations than what previously has been stated to be cytotoxic, and the results show that tyrosinase is not the only cellular target. The cytoskeleton is of utmost importance for the function of all cells and across species. Our data has therefore to be considered in the discussions about the use of hydroquinone for bleaching of skin.