Abstract
Background: Detection of Leishmania antigens in the urine provides a non-invasive means of diagnosis and treatment monitoring of cases of visceral leishmaniasis (VL). Leishmania antigen load in the urine may vary between different time-points within a day, thus influencing the performance of antigen-detection tests. Methods: We investigated the dynamics of Leishmania antigen in urine collected at three different time points (08:00, 12:00 and 16:00 hours). All urine samples collected were tested with the Leishmania Antigen ELISA (VL ELISA) kit, produced by Kalon Biological Ltd., UK. Results: The median concentration of Leishmania antigen in urine collected at 08:00 (2.7 UAU-urinary antigen units/ml) was higher than at 12:00 (1.7 UAU/ml) and at 16:00 (1.9 UAU/ml). These differences were found to be statistically significant (08:00 vs. 12:00, p=0.011; 08:00 vs. 16:00, p=0.041). Conclusion: This pilot study indicates that the Leishmania antigen concentration is higher in urine samples collected in the morning, which has important implications when the VL ELISA kit or other tests to detect Leishmania antigen in urine are used for diagnosis of VL and treatment monitoring.
Funder
Ministerie van Buitenlandse Zaken
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
Reference24 articles.
1. How far are we from visceral leishmaniasis elimination in Bangladesh? An assessment of epidemiological surveillance data.;R Chowdhury;PLoS Negl Trop Dis.,2014
2. Leishmaniasis worldwide and global estimates of its incidence.;J Alvar;PLoS One.,2012
3. Leishmaniasis in high-burden countries: an epidemiological update based on data reported in 2014.;Wkly Epidemiol Rec.,2016
4. Control of the leishmaniases.;World Health Organ Tech Rep Ser.,2010
5. Laboratory diagnosis of visceral leishmaniasis.;S Sundar;Clin Diagn Lab Immunol.,2002
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