Abstract
In the genome of Bombyx mori Linnaeus (1758), the microsatellites, or simple sequence repeats (SSR), feature among their particular characteristics a high adenine and thymine (A/T) content, low number of repeats, low frequency, and a grouping in "families" with similar flanking regions. Such characteristics may be the result of a complex interaction between factors that limit the size and dispersion of SSR loci—such as their high association with transposons—and mean that microsatellites within this taxon suitable as molecular markers are relatively rare. The determination of genetic profiles in populations and cell lines has not been affected owing to the high level of polymorphism, nor has the analysis of diversity, structure and genetic relationships. However, the scarcity of suitable microsatellites has restricted their application in genetic mapping, limiting them to preliminary identification of gene location of genes or quantitative trait loci (QTLs) related to thermotolerance, resistance to viruses, pigmentation patterns, body development and the weight of the cocoon, the cortex, the pupa and the filament. The review confirms that, as markers, microsatellites are versatile and perform well. They could thus be useful both to advance research in emerging countries with few resources seeking to promote sericulture in their territories, and to advance in the genetic and molecular knowledge of characteristics of productive and biological interest, given the latest technological developments in terms of the sequencing, identification, isolation and genotyping of SSR loci.
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
Reference86 articles.
1. Technical manual for tropical sericulture: practical technology to produce silkworm eggs and cocoons in the tropics.;K Sohn;Kigali, Republica de Ruanda: Korea International Cooperation Agency (KOICA).,2014
2. Silkworm, Bombyx mori L. germplasm resources in Bulgaria. National Centre for Agrarian Sciences–Sofia, North-West Regional Agrotechpark - Vratza, Regional Centre for Scientific Applied Service - Vratza, Sericultural Experiment Station–Vratza.;N Petkov,2006
3. From genome to proteome: great progress in the domesticated silkworm (Bombyx mori L.).;Z Zhou;Acta Biochim Biophys Sin (Shanghai).,2008
4. Recent progress in silkworm genetics and genomics;M Goldsmith,2009
5. Microsatellites: evolution and contribution;P Madesis;Methods Mol Biol.,2013
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