Peripheral Blood Mononuclear Cells Regulated Cytokines and Transcription Factors Reduce Haemonchus contortus Larval Establishment in Black Bengal Goat

Abstract

Black Bengal goat (BBG) is a native breed of Bangladesh and its ability in reducing the establishment of H. contortus infection through cellular and immune responses was unexplored. In vitro larval motility in response to PBMC, in vivo larval infectivity assay, differential blood cell counts, histopathology, cytokine and transcription expression have been investigated in BBG kids following H. contortus L₃. In vitro motility experiment revealed that L₃ exposed to PBMC from primed kids had significantly (P < 0.05) reduced speed and straight-line distance compared to L₃ exposed to PBMC from naive kids. Kids with L₃ exposed to PBMC in primed and naive kids, reduced H. contortus egg shed. Upon 10,000 L₃ challenge, WBC count was higher in primed kids compared to naive and 35% of TC were neutrophils. Lymph nodes were increased in weight (P < 0.05) in primed kids except 1st day (D0). IL-4, IL-5, IL-13, IL-33, MCP1, CXCL1, TLR2 and GAL14 were expressed both naive and primed kids. Significant expression of IL-4, IL-5, IL-13, IL-33, MCP1 and CXCL1 at D3, D5 and D7, suggesting early T_H2 differentiation in primed kids. These results suggest that BBG can much resist H. contortus infectivity. Role of transcriptome differences in other resistant breeds should be investigated.