Abstract
Rheumatoid arthritis (RA), an inflammatory illness characterized by joint inflammation and cartilage erosion, currently has limited therapeutic options due to adverse effects. In an experimental model, human dermal fibroblast (HDF) cells expressing miR-192 and platelet-rich plasma (PRP) were applied to alleviate RA symptoms. RT-PCR measured COL1A2 gene expression after transfecting HDF cells and increasing the MIR-192 expression. Male Wistar rats were injected with 100 µl CFA into the foot pad to induce RA. 36 rats were randomly assigned to Model, MTX, Backbone, PRP, miR-192, and PRP + miR-192 groups. Peltysmometers measured paw volume. Standardized arthritis grading was employed. Histopathological changes in ankle joint tissue were investigated using hematoxylin-Eosin staining and serum RF and anti-CCP levels were measured by ELISA. The overexpression of miR-192 in HDF cells increased COL1A2 gene expression 13-fold compared to the control group. PRP and miR-192-expressing HDF cells combination reduced paw volume, arthritic score, serum RF, and anti-CCP. PRP + miR-192 demonstrated less inflammatory cell infiltration and no cartilage damage than other groups. PRP and HDFs expressing miR-192, boosted collagen expression, improved RA symptoms, and prevented cartilage and joint erosion.