Identification of a novel parvovirus in the Arctic wolf (Canis lupus)

Abstract

Abstract Through the utilization of a viral metagenomic approach, a novel virus has been found in a pharyngeal metagenomic library derived from an Arctic wolf (Canis lupus). This virus has been temporarily designated as AWPV and assigned a GenBank accession number BK063423. The genome of AWPV is comprised of 4,920 base pairs, and its nucleotide composition is composed of 36.4% A, 23.4% T, 18.2% G, and 22.0% C, with a GC content of 40.2%. The viral genome demonstrates a typical pattern of parvovirus organization, with two predicted ORFs: ORF1, which encodes non-structural proteins NS1 and NS2, and ORF2, which encodes VP1 and VP2. By performing a pairwise sequence comparison and a phylogenetic analysis based on the NS1 and VP1 protein sequences, it has been suggested that AWPV may represent a novel species within the genus Protoparvovirus. This discovery of a novel parvovirus has enhanced our comprehension of the mammalian virus ecology and has facilitated an improved understanding of potential future infectious diseases.