Affiliation:
1. The First Affiliated Hospital of Zhengzhou University
2. Peking Union Medical College Hospital
Abstract
Abstract
Objective
To analyze the high-throughput sequencing data of giant cell arteritis by bioinformatics technology, to initially identify the core genes associated with giant cell arteritis and to explore potential therapeutic agents.
Methods
Gene expression profile (GSE174694) was obtained from the Gene Expression Database (GEO), and the differential genes were calculated, the differentially expressed genes were analyzed by gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG), and the protein interaction network was constructed to obtain the core genes. Finally, drug correlation analysis (connectivity map, CMap) was used to identify small molecule drugs with potential therapeutic effects on giant cell arteritis.
Results
A total of 771 differentially expressed genes were screened, including 481 up-regulated and 290 down-regulated. The GO analysis showed that the differentially expressed genes were mainly involved in cell surface receptor signaling pathway, T cell receptor signaling pathway, cell adhesion and intrinsic immune response, and the KEGG pathway analysis showed that the differentially expressed genes were mainly involved in chemokine signaling pathway, Th17 cell differentiation and Th1 and Th2 cell differentiation. The KEGG pathway analysis showed that the differential genes were mainly involved in chemokine signaling pathway, Th17 cell differentiation and Th1 and Th2 cell differentiation. The protein interaction network was constructed to screen five core genes, PTPRC, FCGR2B, ITGAM, SPI1 and ITGB2, which were mainly involved in promoting T cell value-added and differentiation, inhibiting apoptosis, increasing cell adhesion and promoting inflammatory response. CMap analysis suggested that small molecules such as warfarin A and anisomycin have potential therapeutic effects on giant cell arteritis. The CMap analysis suggested the potential therapeutic effects of small molecules such as warfarin A and anisomycin on giant cell arteritis.
Conclusion
This study provides a holistic view of the gene transcriptome in giant cell arteritis, and the core genes and small molecule drugs screened may provide new ideas for the pathogenesis of giant cells and drug development.
Publisher
Research Square Platform LLC
Reference34 articles.
1. Systematic review of the literature and a case report informing biopsy-proven giant cell arteritis (GCA) with normal C-reactive protein [J];LARIA A;Clin Rheumatol,2012
2. Tuberculosis Infection in Chinese Patients with Giant Cell Arteritis [J];ZHANG Y;Sci Rep,2019
3. WINKLER A, Giant Cell TRUED. Arteritis: 2018 Review [J]. Missouri medicine, 2018, 115(5): 468 – 70.
4. Giant Cell Arteritis (GCA): Pathogenesis, Clinical Aspects and Treatment Approaches [J];CIOFALO A;Curr Rheumatol reviews,2019
5. Trends in incidence and clinical presentation of temporal arteritis in Olmsted County, Minnesota, 1950–1985 [J];MACHADO E;Arthritis Rheum,1988