Abstract
Single plant root weight, root number, and root length to width ratio are important agronomic traits that affect cassava yield. To explore the QTL related to quantitative traits such as cassava root weight, this study used Hyper-seq simplified resequencing technology to obtain two hybrid progeny from cassava cultivar SC205 as the female parent and cassava cultiva HB60 and cassava cultiva 18R as the male parent, respectively (of which the HB60 × SC205 progeny is called the A1 population, and the 18R × SC205 is called the A2 population). The genotyping of the two F1 mapping populations was conducted, and a genetic map of the maternal parents of the A1 and A2 populations was constructed by comparing the differences between the parental loci and extracting the same variant loci in the offspring and maternal parents. 18 linkage groups were identified for each parent, consistent with the haploid chromosome number of cassava. On average, each parental map had 6,280 markers, with a marker density ranging from 0.37 cM/marker to 0.43 cM/marker. Using the constructed genetic linkage map, QTL analysis of important quantitative traits such as single plant root weight, root number, and root length to width ratio were conducted, and the main QTLs associated with these traits were identified and candidate genes were identified by combining parental transcriptome data. These results help to further validate the use of hyper-seq technology in cassava breeding and provide new methods for developing future varieties.