Affiliation:
1. University of Petroleum and Energy Studies (UPES)
2. Dr. Vishwanath Karad MIT World Peace University
Abstract
Abstract
Hypothyroidism and hyperthyroidism are one of the most frequently observed endocrine disorders in the world. Failure to undergo timely diagnosis and treatment often results in life-threatening events such as arrythmia, hypertension and cardiac arrest. Considering the various limitations of conventional diagnostic techniques, early, accurate and efficient diagnosis of these disorders is pivotal for their timely management. In the present work, a Molecularly Imprinted Polymer (MIP) based sensor is developed for the detection of thyroxine (T4) – the key biomarker. This has been achieved by templating T4 into polyaniline matrix supported on Indium Tin Oxide (ITO) coated glass electrodes. The chemical synthesis of polyaniline was done through oxidative polymerization and optimized using Response Surface Methodology (RSM). The optimum conditions for ultrasonication based extraction of T4 from polyaniline matrix was 15min at 30°C with 75mM NaOH. The developed MIP based sensor was characterized using chromatographic, spectroscopic and electrochemical techniques. The sensor calibration was obtained within T4 concentration range of 5pg/mL – 50pg/mL, with a calculated Limit of Detection (LoD) of7.96pM (6.16pg/mL). The developed MIP-based electroanalytical sensor was found to exhibit high degree of selectivity against various interferants, and thereby possessing huge prospects towards futuristic point-of-care applications.
Publisher
Research Square Platform LLC
Reference36 articles.
1. O. Okosieme Global epidemiology of hyperthyroidism and hypothyroidism;Taylor P;Nat Rev Endocrinol,2018
2. Spencer CA (2000) Assay of Thyroid Hormones and Related Substances. In: Feingold KR, Anawalt B, Boyce A et al (eds) South Dartmouth (MA). MDText.com, Inc.
3. A MoS2@ Ti3C2Tx MXene hybrid-based electrochemical aptasensor (MEA) for sensitive and rapid detection of Thyroxine;Kashefi-Kheyrabadi L;Bioelectrochemistry,2021
4. Pantalone KM, Hatipoglu B, Gupta MK, Kennedy L (2015) (2015) A.H. Hamrahian Measurement of Serum Free Thyroxine Index May Provide Additional Case Detection Compared to Free Thyroxine in the Diagnosis of Central Hypothyroidism. Case reports in endocrinology, 965191
5. Lee Fabrication of electrochemical biosensor composed of multi-functional DNA/rhodium nanoplate heterolayer for thyroxine detection in clinical sample;Park SY;Colloids Surf B: Biointerfaces,2020
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