Abstract
Purpose
This study aims to evaluate SARS-CoV-2 specific IgG (Immunoglobulin G) isolated from post inactive patients as passive immunization candidates.
Patients and methods:
Toxicity testing and virus neutralization parameters. IgG doses of 20, 40, and 60 µg/mL were tested for 3 and 6 days using CPE analysis, viral load, and immunocytochemistry.
Results
Following a 3-days incubation period, the Delta and Omicron variants caused similar levels of damage at 10–20%. However, after 6 days, the CPE (Cytopathic Effect) scoring of the Delta and Omicron variants increased by 70–80% and 50–60%, respectively. The optimal dose for viral neutralization was found to be 40 µg/mL, as it could spread evenly on the actin filament cells, envelop the cells appropriately, and inhibit the virus binding process. The dose was able to reduce virus copy load by up to 26% and 9.63% in the Delta and Omicron variants.
Conclusion
Based on the viral neutralization parameters (Cytopathic Effect, SARS-CoV-2 viral copy load, and immunocytochemistry) showing that the replication process and virulence of the Delta variant were more severe in comparison to the Omicron variant.