Abstract
Background: Due to its increased volume, polycystic ovarian tissue(PCOT) is also more prone to torsion than normal tissue. In treating ovarian torsion, detorsion is applied, and oxygenation of hypoxic tissues is provided with detorsion. The oxygen radicals formed as a result cause tissue damage. Bromelain is a substance obtained from pineapple that can induce apoptosis and free radical formation in macrophages. Aim: This study aimed to evaluate the damage caused to the ovarian tissue by ischemia-reperfusion(I/R) in normal and polycystic ovaries and to investigate the role of bromelain in the damage.
Study Design: Animal experimentation.
Methods: First, PCOT was created by administering dihydroepiandrostenodione-sulfate to 24 rats. Then, the polycystic and normal tissue groups were divided into sham, I/R, and I/R-bromelain groups. After the procedure, all rats' ovarian and tubal tissues were taken and histopathological examination and MDA, TUNEL, NF-κB values were examined.
Results: In this study, it was determined that there was a significant decrease in MDA, NF-κB values, apoptotic-cell rate evaluated by TUNEL in the groups in which bromelain was given in the ovaries of normal and PCOT rats with IR damage, compared to the group that was not provided. It was also shown that I/R damage in PCOT were more significant than in normal ovarian tissue.
Conclusion: The ischemia perfusion damage in polycystic ovarian tissue may be higher than that of normal ovarian tissue. However, there needs to be a literature study investigating this issue. In these respects, this study is the first in the literature. Bromelain is a preferable agent in preventing I / R damage caused by ovarian torsion of PCOT. In addition, it is thought that Bromelain may function in treating ovary torsions, and further studies can be conducted on this subject.