SLC39A14 may be a characteristic gene affecting the development of Barrett's esophagus

Abstract

Abstract Ferroptosis is associated with a variety of pathophysiological processes. The inhibition of ferroptosis has been widely concerned in some diseases. However, no study has yet fully elucidated the role of iron death-related genes (FRGS) in Barrett esophagus. The key genes of ferroptosis in Barrett's esophagus were screened by bioinformatics analysis and verified by experiments. Data were downloaded from the Ferroptosis database (FerrDb) and the Comprehensive Gene Expression Database (GEO) database, and 203 DE-FRGs associated with Barrett's esophagus were obtained, which are associated with immune inflammation, cancer, etc. SLC39A14 was identified as a key gene from these 203 DE-FRGs using SVM-RFE and LASSO algorithms. Functional annotation shows that this gene may have an important impact on Barrett's esophagus through Autophagy animal, HIF-1 signaling pathway, and FoxO signaling pathway and other pathways. Establishing a Barrett's esophagus rat model through “end-to-end anastomosis of esophagus duodenum and preservation of whole stomach”, and detecting the characteristic target SLC39A14 in rat esophageal tissue. And constructing a ceRNA network of characteristic target SLC39A14 related miRNAs and lncRNAs. In summary, this study provides some insights into the pathogenesis of Barrett's esophagus by combining data mining with experimental verification. On the basis of clinical data mining, animal experiments were conducted to verify the key target SLC39A14. It was revealed that SLC39A14 may be a key gene affecting the occurrence and development of Barrett's esophageal disease through ferroptosis pathway, and the miRNA and lncRNA bound by SLC39A14 were predicted.