Extraction of therapeutic protein from Pseudomonas aeruginosa and its cytotoxic effects on cancer cells

Abstract

Abstract In the present study Pseudomonas aeruginosa isolated from immune-compromised patients. P. aeruginosa was identified based on the microscopic observations and biochemical characterization. The Azurin (AZU) gene was exercised form the Genomic DNA of selected P. aeruginosa strain and was cloned in expression vector pMAL-c2X of E.coli BL-21. The cloned cells are screened by blue-white screening method and the expressed azurin after cell lysis was extracted by ammonium precipitation method and purified by dialysis and an ion exchange chromatography techniques. Azurin protein purified was separated and identified by SDS-PAGE. The effect of azurin on proliferation and apoptosis of HCT 15 and T47D cancer cell lines cell line was studied. The results showed that azurin strongly inhibited the proliferation of both colon carcinoma cell line and breast cancer cell line but not on normal cells. These results suggest that r-Azu would be a potential candidate for anticancer drugs and deserves further study.