Cloning of the broad-spectrum wheat leaf rust resistance gene Lr47 introgressed from Aegilops speltoides-Reference-Cited by-同舟云学术

Cloning of the broad-spectrum wheat leaf rust resistance gene Lr47 introgressed from Aegilops speltoides

Published:2023-05-25 Issue: Volume: Page:
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Author:

Li Hongna¹,Hua Lei²,Zhao Shuqing³,Hao Ming⁴^ORCID,Song Rui²,Pang Shuyong²,Liu Yanna²,Chen Hong⁵,Zhang Wenjun⁶,Shen Tao²,Gou Jin-Ying⁷^ORCID,Mao Hailiang⁸,Wang Guiping²,Hao Xiaohua²,Li Jian²,Song Baoxing²,Lan Caixia⁸,Li Zaifeng⁹,Deng Xing Wang¹⁰^ORCID,Dubcovsky Jorge¹¹^ORCID,Wang Xiaodong¹²,Chen Shisheng²^ORCID

Affiliation:

1. Peking University Institute of Advanced Agricultural Sciences

2. National Key Laboratory of Wheat Improvement, Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agriculture Sciences in Weifang

3. State Key Laboratory of North China Crop Improvement and Regulation, College of Plant Protection, Hebei Agricultural University

4. Sichuan Agricultural University

5. Triticeae Research Institute, Sichuan Agricultural University

6. University of California, Davis

7. China Agricultural University

8. National Key Laboratory of Crop Genetic Improvement

9. Agricultural University of Hebei

10. Peking University

11. Howard Hughes Medical Institute and University of California, Davis

12. Hebei Agricultural University

Abstract

Abstract Leaf rust, caused by Puccinia triticinaEriksson (Pt), is one of the most severe foliar diseases of wheat. Breeding for leaf rust resistance is a practical and sustainable method to control this devastating disease. Here, we report the identification of Lr47, a broad-spectrum leaf rust resistance gene introgressed into wheat fromAegilops speltoides. The Lr47 gene encodes a coiled-coil nucleotide-binding leucine-rich repeat protein that is both necessary and sufficient to confer Pt resistance, as demonstrated by loss-of-function mutations and transgenic complementation. New Lr47 introgression lines with no or reduced linkage dragwere generated using the ph1b mutation, and a diagnostic molecular marker for Lr47 was developed. The CC domain of the Lr47 protein was not able to induce cell death, nor did it have self-protein interaction. The cloning of Lr47 expands the number of Pt-resistance genes that can be incorporated into multigene transgenic cassettes to control this devastating disease.

Publisher

Research Square Platform LLC

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