The Placental Bed in Allogenic Pregnancy: Structural and Immunohistochemical Features

Abstract

Abstract Today, ART cycles using a donor egg cell are used more often, but this group of pregnancies has not been sufficiently studied in terms of detecting structural predictors of pregnancy complications caused by a special immunological relationship between the recipient mother and allogeneic blastocytes. A comprehensive clinical and morphological study of 89 allogeneic pregnancies was carried out. The morphological study was performed on placental sites biopsies taken at caesarean section (H & E, Orcein), for immunohistochemical analysis PanCK (AE1 / AE3), SMA (1A4), CD56 (123C3.D5), CD138 (B-A38), CD4 (SP35), CD25 (SP176), CD8 (SP16) were used as primary antibodies. A defect of the remodeling of the spiral arteries, including in subgroups without preeclampsia, the formation of perivascular foci of chronic inflammation were found (the accumulation of CD8 + T lymphocytes, CD56 + NK cells, CD138 + plasma cells, HLA-DR + dendritic cells). In subgroups with PE, significant changes were found for T lymphocytes (51.04 ± 2.3, 55.36 ± 11, 7 versus 37.12 ± 2.3), plasma cells (19.5 ± 3.2, 21.43 ± 8.4 versus 8.94 ± 1.2), Kruskal-Wallace test. Structural and immunohistochemical features of the allogenic placental bed may reflect the complexity of cytotrophoblast invasion and induction of maternal immunity with the formation of immune inflammation.