Assessment of In Vivo and In Vitro Anti-Tumoral Effects of Lycium barbarum Extract on Ehrlich Ascites Tumor Cells : Histopathology, DNA damage and AgNOR

Abstract

Abstract Lycium barbarum has antioxidant and anticarcinogenic effects. Antioxidant activity of extract and its effect on Ehrlich ascites tumor (EAT) was investigated using in vivo and in vitro techniques. EAT cells were injected into Balb/C mice to create a stock mice. EAT cells withdrawn from stock mice were used equal volumes in the studies. In vivo study was consisted control and treatment groups (200 mg/kg fractions above and below 50 kDa of extracts). The liver tissue was evaluated histopathological, DNA damage, and proliferation status. In vitro study was consisted control and treatment groups (1500 and 2000 µg/ml of extracts). Cell viability and apoptosis were evaluated. As a result, decrease in the adhesion of EAT cells, and decreased DNA damage was observed extract group of in vivo study. The extracts both below and above 50 kDa were increased the apoptosis on cancer cells in in vitro study. The extract above 50 kDa were more active than those below 50 kDa. Lycium barbarum consumption may be effective in preventing cancer formation and slowing the progression of cancer.