Aerobic Exercise and Nano-curcumin Supplementation Prevent cancer symptom development through MAPK/ERK pathway

Abstract

Abstract Objective/background: Impaired cell internal settings and its excessive proliferation causes the occurrence of various syndrom and diseases such as cancer. One of the cell signaling pathways that lead to these outcomes is the MAPK/ERK pathway. So, the goal of the present study is to investigate the effect of aerobic training and Nano-curcumin supplementation on RAS and ERK gene expression in rat muscle tissue. Materials and methods In this experimental study, 32 male wistar rats (aged 4–6 weeks, 130–150 g) were randomly assigned into four groups (n = 8/groups), including Control (C), Moderate Intensity Aerobic Exercise (MICT), Nano-Curcumin Supplementation (NCS) and moderate intensity aerobic exercise + Nano-curcumin (MICT + NCS). The training groups implemented the MICT for 4 weeks and 5 sessions per week. The Supplement groups received 80 mg/kg/day through oral gavage for 4 weeks. Genes expression of RAS and ERK were measured with real-time PCR method. Data were analyzed by one-way ANOVA and Bonferroni post-hoc tests (P < 0.05). Findings: Results showed that 4 weeks of moderate intensity aerobic exercise and Nano-curcumin supplementation significantly decreased the gene expression levels of RAS and ERK in muscle tissue of rats. Conclusion Implementation of 4 weeks of MICT combined with Nano-Curcumin supplementation, more efficiently alleviated the RAS/ERK gene expression as symptom development of cancer.