Affiliation:
1. the Fifth Affiliated (Zhuhai) Hospital of Zunyi Medical University
Abstract
Abstract
Background
With the widespread use of antibiotics, more attention has been paid to their side effects. We paid extra attention to the impact of antibiotics on children's bodies. Therefore, we analyzed the characteristic changes in the gut flora of children after antibiotic treatment to explore the pathogenesis of antibiotic-associated diseases in more depth and to provide a basis for diagnosis and treatment.
Methods
We recruited 28 children with respiratory tract infections in the western district of Zhuhai, China, and divided them into three treatment groups based on antibiotic type. We took stool samples from children before and 3-5 days after antibiotic treatment. 16S rRNA gene sequencing was used to analyze the effects of antibiotic therapy on the gut flora of children. Continuous nonparametric data are represented as median values and analyzed using the Wilcoxon rank-sum test.
Results
While alpha diversity analysis found no significant changes in the mean abundance of the gut flora of children after a short course of antibiotic treatment, beta diversity analysis demonstrated significant changes in the composition and diversity of the gut flora of children even after a short course of antibiotic therapy. We also found that meloxicillin sulbactam can inhibit the growth of Proteobacteria, Bacteroidetes, and Verrucomicrobia, ceftriaxone inhibits Verrucomicrobia and Bacteroides, and azithromycin inhibits Fusobacteria, Actinobacteria, Proteobacteria, and Verrucomicrobia. We further performed a comparative analysis at the genus level and found significantly different clusters in each group. Finally, we found that azithromycin had the greatest effect on the metabolic function of intestinal flora, followed by ceftriaxone, and no significant change in the metabolic process of intestinal flora after meloxicillin sulbactam treatment.
Conclusions
Antibiotic treatment significantly affects the diversity of intestinal flora in children, even after a short course of antibiotic treatment. Different classes of antibiotics affect diverse flora primarily, leading to varying alterations in metabolic function. Meanwhile, we identified a series of intestinal flora that differed significantly after antibiotic treatment. These groups of flora could be used as biomarkers to provide an additional basis for diagnosing and treating antibiotic-associated diseases.
Publisher
Research Square Platform LLC
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