Abstract
Background
Severe viral infections can precipitate acute lung injury, causing substantial morbidity and mortality. NETosis plays a crucial role in defending against pathogens and viruses, but its excessive or dysregulated formation can cause pulmonary damage, with research into its regulation offering potential insights and treatment strategies for viral lung injuries.
Methods
Elevated levels of NETosis were detected in the peripheral blood of patients with viral pneumonia. To explore the correlation between NETosis and virus-induced acute lung injury, we employed a murine model, administering poly(I:C) (polyinosinic-polycytidylic acid), an artificial substitute for double-stranded RNA, intratracheally to mimic viral pneumonia. Assessment of NETosis biomarkers in afflicted patients and poly(I:C)-stimulated mice was conducted, alongside mechanistic investigations into the involvement of the Hippo signaling pathway, inflammatory factors, and chemokines in the injury process. Cytokine assays, co-culture experiments, and downstream inflammatory mediator analyses were used to ascertain the role of the Hippo pathway in macrophage to mediate NETosis.
Results
Enhanced expression of NETosis biomarkers was found both in patients with viral pneumonia and in poly(I:C)-stimulated mice. Hippo pathway activation in conjunction with increased levels of inflammatory actors and chemokines was observed in lung tissues of the mouse model. Elevated IL-1β was detected in cells and macrophages isolated from infected mice; this was mitigated by Hippo pathway inhibitors. IL-1β was confirmed to induce NETosis in co-culture experiments, while NLRP3, functioning downstream of the Hippo pathway, mediated its secretion. Patients with viral pneumonia exhibited increased NLRP3 and IL-1β in monocyte-macrophages relative to healthy controls.
Conclusions
Activated Hippo pathway in macrophages during poly(I:C) exposure upregulates NLRP3 and IL-1β expression to promote the occurrence of NETosis, thereby aggravating virus-induced lung injury. This study identifies a potential target pathway for therapeutic intervention to mitigate lung injury stemming from viral infections.