Identification and phylogenetic analysis of R2R3-MYB subfamily in Brassica napus

Abstract

Abstract Background: The majority of the MYB protein's members, plant-specific transcription factors (TFs) that are divided into four classes based on the number of MYB repeats, make up the R2R3-MYB subfamily of proteins. In particular, they play a role in the body's reactions to diverse biotic and abiotic stimuli. R2R3-MYB TFs are involved in physiological and biochemical processes. However, the functions of the rapeseed R2R3-MYBgenes are still mainly unknown (Brassica napus). Results: In this study, a total of 35 BnaMYB genes were found in the rapeseed genome, and details about their physical and chemical characteristics, evolutionary relationships, chromosome locations, gene structures, three-dimensional protein structures, cis-acting promoter elements, and gene duplication were uncovered. The BnaMYB genes had undergone segmental duplications and positive selection pressure, according to the evolutionary study. The same subfamilies have similar intron-exon patterns and motifs, according to gene structure and conserved motif. Additionally, by cis-element analysis, many drought-response and other stress responsive cis-elements were found in the promoter regions of the BnaMYB genes. BnaMYB gene expression displayed a variety of tissue-specific patterns. Twenty of the BnaMYB genes, however, were primarily expressed in stems, therefore 10 lignin-related genes were chosen for drought treatment. Conclusions: Our research screened four genes (BnaMYB7, BnaMYB11, BnaMYB26 and BnaMYB30)showed significant upregulation under drought stress, which may be important drought-response genes. The findings laid a new foundation for understanding the complex mechanisms of BnaMYB in multiple developmental stages and pathways related to drought stress in rapeseed.