Development of SSR markers in Valeriana clarionifolia Phil. and their transferability to other Valeriana spp

Author:

Manifesto Maria Marcela1,Soria Marcelo Abel2,Galvan Fatima Veronica1,Rivero Alexander Gabriel1,Tartara Sabrina Maria Costa3,Arizio Carla Marcela1,Nagahama Nicolas4,Fortunato Renee Hersilia5

Affiliation:

1. CIRN- INTA

2. Universidad Nacional de Buenos Aires

3. Universidad Nacional de Luján

4. Estación Experimental Agroforestal Esquel (INTA)

5. Instituto de Botánica Darwinion (CONICET/ANCEFYN)

Abstract

Abstract Background: Several species from valeriana genus are valuable for their medicinal properties. Due to their economic potential, natural populations are threatened by an indiscriminate harvesting. A profitable plan for conservation and rational use must start with accurate identification and genetic diversity evaluation and tools for these purposes need development for valerian species. In this study, we designed Simple Sequence Repeats (SSR) markers based on transcriptome data for Valeriana clarionifolia Phil. We investigated the polymorphism of these markers and the transferability to five Valeriana species, and we check the applicability in diversity analysis. Methods and Results: We designed 691 markers successfully from 2216 putative simple sequence repeat loci. We isolated 11 SSR markers from 60 selected marker loci and tested their polymorphism in V. carnosa Sm., V. clarionifolia Phil, V. gamainensis N. Nagh.,V. moyanoi Speg, V. philippiana Briq. andV. lapatifolia Vahl. The number of alleles per locus ranged from two to eight and the Polymorphism Information Content varied between 0.29 and 0.77. The loci analysed were successfully cross-amplified in the five congeneric species. Conclusions: This is the first genomic study in native valerians from Patagonia that can be extended to others worldwide Valeriana species. These tools contribute to the identification, conservation, and material selection. In this way, we can attend a severe problem of sustainability production, environmentally respectful to and product quality. Native medicinal plants could turn out in innovative crops for agriculture diversification and a promising alternative for small regional economies.

Publisher

Research Square Platform LLC

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