Extraction, Identification And Determination of Antiviral And Anticancer Flavonoids By HPLC-DAD In Cell Suspension Culture of Melia Azedarach L.

Abstract

Abstract Melia azedarach L. from the Meliaceae, contains a wide range of secondary metabolites used as antibacterial, antiviral, antioxidant and anticancer in traditional medicinal. In the present study, the effect of PGRs and explant type on callus induction and cell suspension culture establishment and growth were investigated. We investigated the biochemical properties, and production and accumulation of secondary metabolites such as rutin, quercetin and kaempferol in M. azedarach L. cell cultures. The results showed that the inflorescence and petiole explants had a high percentage of callus induction compared to the leaf explants, but the highest callus growth were observed in leaf explants in MS+3 mg/L NAA+5 mg/L BAP/3 mg/L Kin and 5 mg/L 2,4-D+5 mg/L Kin (6.307, 5.152 and 3.977 g/explant, respectively). The establishment and growth of cell cultures were significantly influenced by PGRs combination and explant type. The highest cell growth were obtained from the leaf and inflorescence callus transferred into the liquid MS medium supplemented with 1 mg/L 2,4-D+1 mg/L Kin (8.489 g/50 mL suspension) and 1 mg/L 2,4-D+1 mg/L BAP (6.852 g/50 mL suspension), respectively. HPLC analysis showed that the cell cultures derived from inflorescence callus in MS+3 mg/L NAA+1 mg/L BAP showed the highest of rutin (47.536 mg/g FW). However, the highest amount of quercetin (8.570 mg/g FW) and kaempferol (5.420 mg/g FW) were obtained from the petiole cell cultures in the MS+1 mg/L 2,4-D+1 mg/L Kin.