Abstract
Millettia peguensis is a plant native to Southeast Asia belonging to the Fabaceae family. Leaf extracts were prepared using chloroform, methanol, and water. The carrageenan-induced paw edema model in rats was employed to assess anti-inflammatory activity. Paw thickness was measured over time to quantify edema. All extracts exhibited a reduction in paw edema compared to the control group. The water extract demonstrated a significant reduction at the third hour post-injection. Both methanol and chloroform extracts displayed significant inhibition by the fourth hour, but their efficacy was lower than the positive control group treated with indomethacin. By the fifth hour, both water and methanol extracts produced results comparable to indomethacin. The study assessed DPPH antioxidant activity of extracts from using chloroform, methanol, and distilled water. Methanol and distilled water extracts showed higher antioxidant activity compared to chloroform extract, suggesting the significance of polar compounds. The findings highlight the impact of solvent choice on extracting bioactive antioxidants. Phytochemical analysis suggested the presence of bioactive compounds such as flavonoids, tannins, saponins, and phenolic compounds. Methanol and water extracts showed higher antioxidant activity due to the presence of phenolic compounds, which may be responsible for the observed anti-inflammatory activity.