An automated workflow for the rapid increase of hydrolytic enzyme concentration for short- chain carboxylic acid production from lignocellulosic biomass

Author:

Longis Marion1,Raulo Roxane2,Paul Sébastien3,Neubauer Peter1,Phalip Vincent2,Heuson Egon3,Junne Stefan1

Affiliation:

1. Technische Universität Berlin

2. UMRT BioEcoAgro 1158, Univ. Lille, INRAE, Univ. Liège, UPJV, JUNIA, Univ. Artois, Univ. Littoral Côte d’Opale, ICV – Institut Charles Viollette

3. Univ. Lille, CNRS, Centrale Lille, Univ. Artois, UMR 8181–UCCS—Unité de Catalyse et de Chimie du Solide

Abstract

Abstract Background. Short-chain carboxylic acids are interesting building blocks that can be synthesized from biogenic residues with the so-called dark fermentation. One challenge though is the hydrolysis of lignocellulosic residues to make them accessible for whole cell biotransformation. Accessibility can be achieved through conversion of lignocellulose through microbial exoenzymes. In this study, bacteria and fungi were isolated from different lignocellulose-containing feedstock. A workflow of an automated screening with the isolated strains for their ability to accumulate short-chain carboxylic acids in fermentation was elaborated. The screening was performed with and without addition of fungal enzymes. Results. Results show a three-fold increase in acetic acid concentration and nearly a six-fold increase in succinic acid concentration if the feedstock was pre-treated in comparison to untreated feedstock. When adding the enzyme-containing supernatant to a continuous dark fermentation with wood barks as feedstock, the acid concentration increased remarkably from 1 to 2.6 g⋅L− 1. Conclusion. The methodology, thus, is suitable to identify strains and enzyme mixtures for improving hydrolysis of complex lignocellulosic feedstock.

Publisher

Research Square Platform LLC

Reference46 articles.

1. Lignocellulosic biomass: a sustainable platform for the production of bio-based chemicals and polymers;Isikgor FH;Polym Chem,2015

2. Dence CW. The determination of lignin. Methods Lignin Chem Springer Ser Wood Sci (Lin,SY Dence,carlt W, eds). Springer B. Berlin, Heidelberg; 1992. p. 33–61; doi: ໿10.1042/bj0282160

3. Cao X, Ding B, Yu J, Al-Deyab SS. Cellulose nanowhiskers extracted from TEMPO-oxidized jute fibers. Carbohydr Polym. 2012;90:1075–80; doi: ໿10.1016/j.carbpol.2012.06.046

4. Dahiya S, Lakshminarayanan S, Venkata Mohan S. Steering acidogenesis towards selective propionic acid production using co-factors and evaluating environmental sustainability. Chem Eng J. 2020;379:122135; doi: ໿10.1016/j.cej.2019.122135

5. Patel A, Sarkar O, Rova U, Christakopoulos P, Matsakas L. Valorization of volatile fatty acids derived from low-cost organic waste for lipogenesis in oleaginous microorganisms-A review. Bioresour Technol. 2021;321:124457; doi: ໿10.1016/j.biortech.2020.124457

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